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KMID : 0615720160330030101
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Jouranl of Korean Acupuncture and Moxibustion society
2016 Volume.33 No. 3 p.101 ~ p.116
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Effects of Ukgansan Pharmacopuncture at GB20 on Cognitive Impariment Induced by Focal Brain Injury in Rats
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Lee Jung-Hun
Yang Tae-Jun
Jeong Sang-Jun
Wei Tung-Sheun
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Abstract
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Effects of Ukgansan Pharmacopuncture at GB20 on Cognitive Impariment Induced by Focal Brain Injury in Rats Jung Hun Lee, Tae Jun Yang, Sang Jun Jeong and Tung Shuen Wei* Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University
Objectives : This research was performed to investigate the effects of Ukgansan pharmacopuncture( U-PA) of focal brain ischemia induced by middle cerebral artery occlusion(MCAO) in rats. Methods : The subjects were divided into 5 groups : A control group, acupuncture group, pharmacopuncture group U-PA1(2.571 mg/ 250 g/ 40 ㎕), pharmacopuncture group U-PA2(6.428 mg/ 250 g/ 40 ㎕), and pharmacopuncture group U-PA3(12.855 mg/ 250 g/ 40 ㎕). The focal brain ischemia was induced by intraluminal filament insertion into the middle cerebral artery. After 3 days of MCAO, Ukgansan(UGS) pharmacopuncture treatment was performed on the GB20, and the day after being treated with pharmacopuncture, the Morris water maze test was carried out by the assigned group. The series of processes were treated 6 times. Thereafter Bax, Bcl-2, Bax/Bcl-2 ratio, mGluR5, density of neuronal cell, and ChAT were measured.
Results : The results were as follows. 1. The intensity of Bax significantly decreased in the U-PA1, U-PA2, U-PA3 groups. 2. The Bax/Bcl-2 ratio significantly decreased in the U-PA3 group compared with the control group. 3. The neuroprotective effect on the hippocampal CA1 significantly increased in the U-PA1, U-PA2, U-PA3 groups compared with the control group. 4. The density of ChAT in the hippocampal CA1 significantly increased in the U-PA1, U-PA2, U-PA3 groups compared with the control group.
Conclusion : These results suggest that UGS pharmacopuncture may have anti-apoptotic and neuroprotective effects on focal cerebral ischemia caused by intraluminal filament insertion into the middle cerebral artery in rats.
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KEYWORD
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Vascular dementia, MCAO, Ukgansan, Pharmacopuncture, GB20
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